Development of an economical fermentation platform for enhanced ansamitocin P-3 production in Actinosynnema pretiosum
نویسندگان
چکیده
منابع مشابه
An integrative expression vector for Actinosynnema pretiosum
BACKGROUND The Actinomycete Actinosynnema pretiosum ssp. auranticum has commercial importance due to its production of ansamitocin P-3 (AP-3), a potent antitumor agent. One way to increase AP-3 production would be to constitutively express selected genes so as to relieve bottlenecks in the biosynthetic pathway; however, an integrative expression vector for A. pretiosum is lacking. The aim of th...
متن کاملThe biosynthetic gene cluster of the maytansinoid antitumor agent ansamitocin from Actinosynnema pretiosum.
Maytansinoids are potent antitumor agents found in plants and microorganisms. To elucidate their biosynthesis at the biochemical and genetic level and to set the stage for their structure modification through genetic engineering, we have cloned two gene clusters required for the biosynthesis of the maytansinoid, ansamitocin, from a cosmid library of Actinosynnema pretiosum ssp. auranticum ATCC ...
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15 صفحه اولUnprecedented deoxygenation at C-7 of the ansamitocin core during mutasynthetic biotransformations
We describe the unprecedented formation of six ansamitocin derivatives that are deoxygenated at C-7 of the ansamitocin core, obtained during fermentation experiments by employing a variety of Actinosynnema pretiosum mutants and mutasynthetic approaches. We suggest that the formation of these derivatives is based on elimination at C-7/C-8 followed by reduction(s) of the intermediate enone. In bi...
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ژورنال
عنوان ژورنال: Bioresources and Bioprocessing
سال: 2019
ISSN: 2197-4365
DOI: 10.1186/s40643-018-0235-3